Page 1 of 3
X-ray crystallography has long been the most potent method for obtaining high-resolution structural information on proteins, nucleic acids and their complexes. In 2014, 9628 entries were added to the Protein Data Bank (PDB) to bring the total count of released structures above 100,000. In 2015 this success continued, with again more than 9000 structures released throughout the year. But it was not just the quantity of the structures. The increasing quality is something to celebrate as well.
In 2015, 148 papers in the three journals with the broadest scientific reach (Cell, Science and Nature) presented results obtained with contributions from macromolecular x-ray crystallography. These papers contained 440 crystal structures of biologically important systems, from antibodies against human pathogenic viruses (Hashiguchi et al., Impagliazzo et al., Robinson et al., Rouvinski et al., Scharf et al., Wu et al., Zhou et al.) to important trans-membrane transporters (Arakawa et al., Deng et al., Kato et al., Leung et al., Lin et al., Moser von Filseck et al., Nomura et al., Perez et al., Tao et al., Wang et al.) and proteins involved in membrane fusion (Diao et al., Rostislavleva et al., Zhou et al.). We would like to congratulate all involved researchers on their achievements.
Figure 1. Cartoon representations of three membrane protein structures solved with PILATUS data. Shown are, from left to right, the anion exchanger domain of human erythrocyte band 3 (PDB code 4yzf), the mammalian fructose transporter GLUT5 (PDB code 4yb9) and transhydrogenase (PDB code 4o93).
At DECTRIS, we are proud that data from PILATUS detectors is over-represented among the highest-impact research compared to the PDB overall. Whereas 23% of the coordinate sets released in 2015 were obtained from data collected on PILATUS detectors, 37% of PDB entries published in Cell, Science and Nature were based on PILATUS data. Forty-seven of the structures reported last year in these three high-impact journals were solved by experimental phasing. In this subset where the highest data quality is essential, the share of structures solved from PILATUS data is nearly 43%. Our customers know that better detectors will help them obtain better data.
It is thus not surprising that we ship PILATUS3 and its successor, EIGER, to an increasing number of beamlines at synchrotrons worldwide. In 2015, we installed a PILATUS3 S 2M at beamline 14-2 of Bessy II in Berlin, Germany, and a PILATUS3 S 6M at sector 5 of ALS in Berkeley, California. We also delivered one PILATUS3 X 6M detector each to SBC-CAT at APS in Argonne, Illinois, and to beamline 11C of PLS in Pohang, Korea, but the big news for crystallographers was the arrival of EIGER at the first synchrotron facilities.
In May, two EIGER X 4M detectors were installed at beamline BL-1A of Photon Factory in Tsukuba, Japan, in a V-shaped conformation inside a helium-filled chamber. This unusual setup will facilitate the collection of high-quality anomalous diffraction data at x-ray energies as low as 3.7 keV. The first EIGER X 16M, our new flagship detector, entered service at beamline X06SA of SLS in Villigen, Switzerland, in October.
In the two months that remained of the year, an EIGER X 16M was installed at beamline FMX of NSLS II in Brookhaven, New York, an EIGER X 9M at beamline Proxima-2A of Soleil in Saclay, France, and another EIGER X 9M at LS-CAT at APS. The EIGER X 9M at Proxima-2A produced data to solve the first novel protein structure less than a month after arrival of the detector onsite.
The future is bright for structural biology. Cryo-electron microscopy has just been named Method of the Year by the editors of the Nature journals. Electron microscopy and crystallography will be increasingly synergistic in resolving challenging targets, but crystallography is still growing and will dominate structural biology for most biological systems for the foreseeable future.
DECTRIS is pleased to announce the partnership with Titan Electro-Optics Co., Ltd. (TEO) as one of our main distributor for sales and support in China.
With TEO, DECTRIS gains a competent partner specialized in laser and spectroscopy applications and system integration equipment for scientific and industrial use with a high level of expertise in Tokamak applications.
TEO has long-term experiences as a distributor of high performance cameras, such as Andor Technology, Radiant VS or PKI. TEO's sales activities will focus on the non-exclusive distribution of DECTRIS detectors and extend the support for DECTRIS X-ray detectors in China. The distributor contract was signed by Jean-Dong, Head of Industrial Photonics Division at TEO and Ruben Vogelsang, Head of Sales at DECTRIS.
With this partnership, DECTRIS is strengthening its presence around the world to provide optimal service for our customers. It is furthermore the next step in DECTRIS’ way to increase its presence and sales in China.
Villigen, November 2015: The macromolecular crystallography beamline X06SA at the Swiss Light Source (SLS), a synchrotron operated by Paul Scherrer Institute (PSI), is the first one in the world to upgrade its detector to an EIGER X 16M. On 14 Oct 2015, the new detector entered service, and it is already a success. Meitian Wang, group leader and beamline responsible, is delighted with the new detector's stability. "Since its installation last month, the EIGER has operated without any problems", he said.
The EIGER X 16M succeeds a PILATUS 6M, an instrument that has led to the determination of many important protein structures and contributed to the reputation of beamline X06SA as one of the world's best. In addition to the technical specs, Wang is also excited about is the application programming interface that simplifies detector operation. The transition from the camserver of the PILATUS to the new API was a happy one.
Firmware version 1.5 delivered with the EIGER represents a very stable and high-performance release. "The system installed at SLS exceeds the specifications that we promised during the Synchrotron Radiation Instrumentation conference in July 2015", said Clemens Schulze-Briese, CSO of DECTRIS.
In tests before EIGER went online, Wang and colleagues collected data from crystals whose longest unit cell dimension exceeded 1000 Å. Thanks to EIGER's pixel size of only (75 µm)2, data could have been collected to well beyond the resolution limit of the crystal of 3 Å. More relevant for regular users will be the continuous read-out feature, which ensures duty cycles of above 99% even when the detector runs at full speed and makes breathtakingly fast experiments possible. In separate tests, Wang was able to successfully collect an entire S-SAD dataset within one second.
Among the first users of the EIGER X 16M was Hauke Hillen, a PhD student from the Max Planck Institute for Biophysical Chemistry in Göttingen. On a recent afternoon, he was busy collecting data for experimental phasing of a crystal structure based on the sulfur signal. Between datasets, he stopped for a quick chat: "I'm very impressed with the EIGER", he said. "It's so fast." With this, the conversation was over, as he had to rush back to his experiment.
The high frame rate and large number of pixels of the EIGER X 16M lead to unprecedented amounts of raw data. To make online data processing, speedy transfer to the users' home labs and reliable archiving possible, bit-shuffle filtered LZ4 compression was introduced. This compression strategy is not only fast enough to keep up with the generated data but also more efficient than CBF compression. Vincent Olieric, beamline scientist at the PSI, was impressed that the new detector does not generate much more data on disk than the previous PILATUS 6M. "It's great for users", he said.
The development of the EIGER X 16M now operating at SLS was accelerated by a grant by the Swiss Commission for Technology and Innovation (CTI) to promote collaboration between academia and business. The detector could thus be tested under realistic experimental conditions from an early stage. Moving from prototype to final product, DECTRIS engineers and scientists have developed an instrument that meets the needs of the most exacting X-ray crystallographers. Eventually, all EIGER customers will benefit from that.
About DECTRIS Ltd.
DECTRIS Ltd. is the technologically leading company in Hybrid Photon Counting X-ray detection. DECTRIS Hybrid Photon Counting detectors have transformed basic research at synchrotron light sources, as well as in the laboratory and with industrial X-ray applications. DECTRIS aims to continuously improve the measurement quality, thereby enabling new scientific findings. A broad range of products is based on this pioneering technology; all scaled to meet the needs of various applications. DECTRIS also develops bespoke solutions for scientific and industrial X-ray detection.
The Paul Scherrer Institute (PSI) develops, builds and operates large, complex research facilities and makes them available to the Swiss and international research community. The institute's own key research priorities are in the fields of matter and materials, energy and environment and human health. PSI is committed to the training of future generations. Therefore about one quarter of the staff are post-docs, post-graduates or apprentices. Altogether PSI employs 1900 people, thus being the largest research institute in Switzerland. The annual budget amounts to approximately CHF 350 million.EIGER X 16M @ SLS
EIGER X 16M at Swiss Light Source beamline X06SA by Oliver Bunk, Paul Scherrer Institute.
Individual diffraction spots resolved for crystal with unit cell of 1000 Å by Arnau Casañas, Paul Scherrer Institute.
Karlsruhe, Germany and Baden, Switzerland, September 2, 2015 — Bruker AXS and DECTRIS Ltd. today announced an agreement for the supply of Hybrid Photon Counting (HPC) pixel detectors for Bruker’s X-ray diffraction (XRD) business.“We have chosen DECTRIS as a supplier because of its high-performance, reliable, and proven HPC detector technology and high-volume production capacity”, says Frank Burgaezy, President of Bruker AXS. “The combination of our world-class technologies will benefit customers in a wide range of demanding applications such as micro-high-resolution diffraction (HRXRD), grazing incidence small angle X-ray scattering (GISAXS) and kinetic studies’’“DECTRIS is pleased to supply state-of-the-art HPC detectors to Bruker, a global leader in X-ray technology with a long tradition of innovation”, states Christian Broennimann, CEO of DECTRIS. “We are excited by the performance of our noise-free PILATUS3 detector combined with the cutting-edge D8 DISCOVER diffraction solutions.” The PILATUS3 R 100K-A 2D X-ray detector for the D8 DISCOVER diffraction solutions was announced today at the Japan Analytical & Scientific Instrument Show 2015 (JASIS). About Bruker AXS:Bruker AXS, a division of the Bruker Corporation (BRKR), is a global market and technology leader in materials research, life science and quality control instrumentation for elemental and crystalline structure investigations. The solutions cover bulk material and surface sensitive X-ray diffraction, biological and chemical crystallography, wavelength and energy dispersive X-ray fluorescence analysis, optical emission spectroscopy, and combustion analysis. Learn more about Bruker AXS at www.bruker.com.About DECTRIS Ltd.DECTRIS is the technology leader in Hybrid Photon Counting X-ray detection. Based in Baden, Switzerland, the company has all the required experiences and resources for the design, production and global distribution of its detectors. The DECTRIS photon counting detectors have transformed basic research at synchrotron light sources, as well as in the laboratory and with industrial X-ray applications. DECTRIS aims to continuously improve the measurement quality, thereby enabling new scientific findings. This pioneering technology is the basis of a broad range of products, all scaled to meet the needs of various applications. DECTRIS also provides solutions for customer developments in scientific and industrial X-ray detection.
Villigen, March 2015: Researchers at the Swiss Light Source, a synchrotron radiation facility at the Paul Scherrer Institute (PSI) in Villigen, have developed a new approach to solving crystal structures from unmodified protein and nucleic acid crystals. Traditionally, the determination of a new protein structure depended on the calculation of the positions of exogenous heavy atoms whose incorporation is often laborious or inefficient.
Using a DECTRIS PILATUS 2M detector, PSI researchers improved a method known as “native SAD” (single-wavelength anomalous diffraction) where only atoms present in the protein are used to determine the structure. Until now, this method was limited to structures of small, well-diffracting proteins. With the new approach of collecting low-dose data at high redundancy, in multiple sweeps with the crystal in multiple orientations, the weak signal inherent in light atoms naturally occurring in protein crystals (e.g. sulfur, calcium, potassium) can be extracted reliably, in particular since radiation damage is minimized.
The high sensitivity of the PILATUS detectors produced by DECTRIS was critical to the success of the new approach. “These detectors can record signals with low noise with a low intensity of X-ray light, which means one can determine the protein structure with a low X-ray dose,” explains Vincent Olieric, a scientist at PSI who was involved in the project. With the new, broadly applicable technique, five new structures, among them challenging targets like a human membrane protein, a protein-DNA complex and a large multiprotein-ligand complex, were solved from native protein crystals (Weinert et al., Nature Methods 12, 131–133, 2015).
About DECTRIS Ltd.DECTRIS Ltd. is the technologically leading company in Hybrid Photon Counting X-ray detection. DECTRIS Hybrid Photon Counting detectors have transformed basic research at synchrotron light sources, as well as in the laboratory and with industrial X-Ray applications. DECTRIS aims to continuously improve the measurement quality, thereby enabling new scientific findings. The broad range of products is based on this pioneering technology; all scaled to meet the needs of various applications. DECTRIS also provides solutions for customer developments in scientific and industrial X-Ray detection.
About PSIThe Paul Scherrer Institute PSI develops, builds and operates large, complex research facilities and makes them available to the national and international research community. The institute's own key research priorities are in the fields of matter and materials, energy and environment and human health. PSI is committed to the training of future generations. Therefore about one quarter of our staff are post-docs, post-graduates or apprentices. Altogether PSI employs 1900 people, thus being the largest research institute in Switzerland. The annual budget amounts to approximately CHF 350 million.
Hybrid Photon Counting (HPC) pixel detectors enable noise-free X-ray imaging with high dynamic range and a sharp point-spread function. Their excellent stability at high frame rates in combination with the absence of image lag is essential for superior data quality. The new EIGER detector (75 µm) has been tested for high-resolution grating-based phase-contrast imaging. CdTe-based PILATUS3 detectors (172 µm) for hard X-ray imaging, up to 100 keV, have been developed and tested for spectral imaging.
Baden/Graz, Januar 2015: Petra Kotnik, Department Manager of Material Characterization at Anton Paar GmbH, and Clemens Schulze-Briese, CSO at DECTRIS Ltd., have agreed to integrate the new EIGER R 1M detector in the SAXSpace laboratory SWAXS system from Anton Paar.
The EIGER R 1M is DECTRIS latest Hybrid Photon Counting (HPC) detector for laboratory applications. Its outstanding features convinced Anton Paar to offer the novel detector with their excellent SAXSpace system.
Heiner Santner, Product Manager for SAXS products at Anton Paar, states: “DECTRIS HPC detectors are renowned for their single-photon sensitivity. In addition, the new EIGER R 1M combines small pixel size with a sharp point-spread function to achieve exceptional resolution. We make use of of the EIGER 2D detector’s advantages for fast and accurate analysis of nanostructured materials in our SAXSpace system. Scientists benefit from a large detector area, excellent resolution and unmatched readout speed and data accuracy.”
“Being our first partner to sign a frame contract for EIGER detectors shows that Anton Paar is dedicated to providing their customers with state-of-the-art technology in cutting-edge instruments,” says Clemens Schulze-Briese. “We are happy that Anton Paar fully integrates EIGER detectors into its outstanding SWAXS systems that are easy to use and that make the most out of the potential of our detectors for the users.”
About DECTRIS Ltd.DECTRIS Ltd. is the technologically leading company in Hybrid Photon Counting X-ray detection. DECTRIS Hybrid Photon Counting detectors have transformed basic research at synchrotron light sources, as well as in the laboratory and with industrial X-Ray applications. DECTRIS aims to continuously improve the measurement quality, thereby enabling new scientific findings. The broad range of products is based on this pioneering technology; all scaled to meet the needs of various applications. DECTRIS also provides solutions for customer developments in scientific and industrial X-Ray detection. DECTRIS was awarded the 2010 Swiss Economic Award in the High-Tech Biotech category, the most prestigious prize for start-up companies in Switzerland.About Anton PaarAnton Paar develops, produces and distributes highly accurate laboratory instruments and process measuring systems, and provides custom-tailored automation and robotic solutions. Anton Paar is the leading company for density and concentration measurement, rheometry and X-ray structure analysis (SAXS and non-ambient attachments for XRD). Anton Paar GmbH is owned by the charitable Santner Foundation.
November 2014, Zurich, Switzerland: Christian Brönnimann, CEO of DECTRIS, proudly accepted the Swiss Excellence Product Award of 2014 for the new X-ray detector series EIGER. Launched in August 2014 at the IUCr Conference in Montreal, Canada, the EIGER X-ray detector series was awarded as an innovative and unique development for advanced X-ray diffraction analysis at synchrotron beamlines as well as in the laboratory. The high resolution and the noise-free detection in combination with ultimate speed enable the user to take highly detailed diffraction images in a quality that could never be reached before.
The yearly Swiss Excellence Award is presented to manufacturers with outstanding innovations, high quality products and technological value added.
The delegates received ten days of intensive training by STOE, DECTRIS and Xenocs staff as well as guest scientists in cooperation with the IUCr.
In the first lecture, DECTRIS introduced the delegates to the instrumentation for X-ray analysis: from sealed X-ray tubes and photographic films to 4th generation synchrotrons and modern detectors. Advances of single-photon-counting technology in laboratory and at synchrotron facility were presented on several examples of difficult structures, whose solution was possible only owing to the features of MYTHEN and PILATUS detectors.
In Grenoble, the delegates received theoretical and practical training on Small Angle X-ray Scattering. Four different workshops were organized at Xenocs Headquarters so that the participants could get in-depth knowledge on Xenocs sources and optics, Xenocs SAXS/WAXS systems and data treatment software, with experiments carried out on the Xeuss 2.0 SAXS/WAXS system. The delegates also visited the European Synchrotron Radiation Facility (ESRF) and more specifically the ID02 SAXS beamline.
In Darmstadt, the participants were trained in single crystal and powder XRD at STOE’s labs. The training involved one day of theoretical lectures, while two full days focused on hands-on workshops for x-ray single crystal as well as x-ray powder diffraction on the STOE IPDS 2/2T and STADIVARI Single Crystal Diffractometer, and STADI P and STADI MP Powder Diffractometer. Thereafter, the participants joined STOE’s Single Crystal User Meeting, which included several talks from guest scientists and STOE staff. As a highlight, Prof. Dr. Gautam R. Desiraju, immediate past president of the IUCR, has held his premier speech on “100 years of Crystallography: Seen through 15 small molecule crystal structures”.
The feedback of the participants was overwhelming. Next to all the knowledge being shared, it has been impressive how strong ties have been built among the participants – connecting young crystallographers from across the world in the spirit of IYCr.
Read more at: http://www.chemistryviews.org/details/ezine/6749191/IUCr-UNESCO_OpenFactory.html
Kyoto (Japan), June 2014: Hiroyuki Murakita, General Manager of X-ray/Surface Analysis Business Unit at SHIMADZU Corporation and Clemens Schulze-Briese, CSO at DECTRIS Ltd. signed a frame contract about the delivery of detectors for the x-ray diffraction systems of SHIMADZU.
Due to the outstanding reputation of DECTRIS detectors in the X-ray diffraction market, SHIMADZU signed a delivery agreement for detectors for their X-ray diffraction systems. Hiroaki Furukawa, Product Manager for X-ray products at Shimadzu says: “The detector has 1280 channels. We can use this point to realize the faster analysis of our XRD customers for various applications, such as the quality control of chemical materials including pharmaceutical, the analysis of catalyst, and environmental applications. We developed the software for controlling this detector and succeeded to maximize the performance of the detectors.”
Clemens Schulze-Briese says: “Our knowledge in the synchrotron market enables us to provide high reliable and sensitive detectors for the laboratory market. We believe that the customers receiving the systems from SHIMADZU including our detectors will be very satisfied and happy to work with such a high performance X-ray diffractometer”
Analytical & Measuring Technology developed by Shimadzu Corporation can be easily termed as "Technology for Science". Our high-level, sophisticated products include Chromatographs, Light Absorption Analysis Systems, and Composition and X-ray/Surface Analysis Systems. We provide a broad range of analytical instruments indispensable for research, development, and quality control in a variety of fields, including new drug development. For primary instruments such as the Liquid Chromatographs and Gas Chromatograph Mass Spectrometers, we have moved actively to incorporate technology that responds to the latest global needs. As a result, we are anticipating continued future growth in these important markets. Shimadzu also provides a broad range of high-precision testing and measuring technology that is essential for product development. Our high-precision measuring instruments are used for the evaluation of physical properties, non-destructive inspection, and environmental measurement.